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PHRED Photochemical Reactor

 

Photochemical Reactor for Enhanced Detection (PHRED) is a compact unit placed between the HPLC column and the detector to perform on-line post-column continuous photolytic derivatization to increase the sensitivity and selectivity of response of fluorescence, ultraviolet, electrochemical, and chemiluminescence detectors.

PHRED consists of a lamp holder, a 254 nanometer low pressure mercury lamp, and a holder for the knitted reactor coils (KRCs). The KRCs are especially designed to fit into "PHRED" and their material of construction (PTFE) is transparent to the 254 nm uv light.

PHRED is often used in the determination of aflatoxins by reverse phase HPLC with fluorescence detection.  PHRED is a also a versatile unit that has been successfully employed in the determination of hundreds of other compounds including niacin and niacinamide.

 

PHRED photochemical reactorPHRED photochemical reactor PHRED and the stainless steel supporting plate.

 

Advantages of using PHRED:

  1. Approved by all major regulatory organizations for aflatoxin analyses
  2. Low initial cost
  3. Low maintenance
  4. Low cost for consumables
  5. High sensitivity, reproducibility and reliability
  6. Eluents require no dissolved salts, last indefinitely, do not degrade pumps, require less technician time for preparation and can be recycled
  7. No moving parts except photons
  8. Small footprint
  9. Allows simultaneous analysis of additional mycotoxins
  10. PHRED can be used for many other applications as listed in the extensive bibliography listed below
  11. Available in 100, 120, and 230 volt models

 

PHRED Catalog Numbers and Description:

Catalog No. Description
PHRED-8 Photochemical reactor unit with one 254 nanometer low pressure mercury ultraviolet bulb, one 8” polished support plate, and a package of 10 nylon ties. For 120 VAC.
PHRED-12 As above, with 12" polished support plate.
PHRED-CE-8 As above, with 8" polished support plate.  For 230 VAC.
PHRED-CE-12 As above, with 12" polished support plate.  For 230 VAC.
PHRED-100-8 As above, with 8" polished support plate.  For 100 VAC. (Japan)
PHRED-100-12 As above, with 12" polished support plate.  For 100 VAC (Japan)
BULB-254 254 nanometer ultraviolet bulb.
BULB-366 366 nanometer ultraviolet bulb.
PSP-8 8" polished stainless steel support plate.
PSP-12 12" polished stainless steel support plate.
NT-10 Nylon ties (package of 10)
PHRED photochemical reactor

 

Official Methods of Analysis

Analysis of Aflatoxin using HPLC with Post-Column Photochemical Derivatization:

  • Recommended Practice Aa 11-05 (Approved 2005), Official Methods and Recommended Practices of the AOCS Fifth Edition, Second Printing.
  • AOAC Official Method 2005.08, section number 49.2.18A
  • Aflatoxins in Corn, Raw Peanuts and Peanut Butter using Liquid Chromatography with Post-Column Photochemical Derivatization.
  • Official Methods of Analysis of AOAC INTERNATIONAL (2005) 18th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, Official Method 2005.08

Analysis of Niacin using HPLC with Post-Column Photochemical Derivatization:

  • European Committee for Standardization. Foodstuffs – determination of niacin by HPLC. EN 15652:2009, 2009.

Analysis of NDELA using HPLC with Post-Column Photochemical and Chemical Derivatization:

  • ISO 10130:2009 Cosmetics- Analytical methods – Nitrosamines: Detection and determination of N-nitrosodiethanolamine (NDELA) in cosmetics by HPLC, post-column photolysis and derivatization.

 

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  105. Schulte-Ladbeck,P. Kolla, and U. Karst. Trace Analysis of Peroxide-Based Explosives, Analytical Chemistry, 75: 731-735 (2003)
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  107. Selavka, C.,I. Krull, and K. Bratin. Analysis for Penicillins and Cefoperazone by HPLC-Photolysis­ Electrochemical Detection, Journal of Pharmaceutical and Biomedical Analysis, 4: 83-93  (1986)
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