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Economical Post Column Derivatization Kit (EPOCOD)

 

Economical Post Column Derivatization Kit (EPOCOD) is an economical and compact unit placed between the HPLC column and the detector designed to facilitate the convenient mixing of the analyte containing eluent from the HPLC column with chemical derivatization agents. The kit includes a PTFE knitted reactor coil, a column jacket for temperature control and a “T” connector for convenient mixing of the analyte with post column chemical derivatization agents.

A standard EPOCOD comes with a knitted reactor coil 5 meters long x 0.5 mm ID in a 4” long column jacket (EP B-4-5-50).   Other combinations of knitted reactor coils and column jackets can be provided based on your specific analytical needs.  A water circulator is used in conjunction with the column jacket to control the temperature of the post column chemical derivatization reaction. 

 

EPOCOD Economical Post Column Derivatization Kit (EPOCOD)

 

EPOCOD Catalog Numbers and Description:

Catalog No. Knitted Reactor Coil Length (M) ID (mm) Nominal Void Volume (mL) Recommended Column Jacket
EP B-4-5-25 KRC 5-25
5
0.25
0.25
CJB-4
EP C-10-10-25 KRC 10-25
10
0.25
0.5
CJC-10
EP C-10-15-25 KRC 15-25
15
0.25
0.75
CJC-10
EP C-10-20-25 KRC 20-25
20
0.25
1
CJC-10
EP C-10-25-25 KRC 25-25
25
0.25
1.25
CJC-10
EP B-4-5-50* KRC 5-50
5
0.5
1
CJB-4
EP C-10-10-50 KRC 10-50
10
0.5
2
CJC-10
EP C-10-15-50 KRC 15-50
15
0.5
3
CJC-10
EP C-10-20-50 KRC 20-50
20
0.5
4
CJC-10
EP C-10-25-50 KRC 25-50
25
0.5
5
CJC-10
Economical Post Column Derivatization Kit (EPOCOD)

* Standard

 

Bibliography

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  69. Kawamoto, T., Okada, E., Fujita, T. Post-Column Derivatization of Vitamin B6 using 2-6-dibromoquinone-4-chlorimide J. CHROMATOG. 267, 414-419 (1983)
  70. Hunter, K. Determination of Coumarin Anticoagulant Rodinticide Residues in Animal Tissue by HPLC. 1. Fluorescence Detection using Post-Column Techniques. J. CHROMATOG. 270, 267-276 (1983)
  71. Honda, S., Takahashi, M., Araki, V., Kakehi, K. Post-Column Derivatization of Catecholamines with 2-Cyanoacetamide for Fluorimetric Monitoring in HPLC. J. CHROMATOG. 274, 45-52 (1983)
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  73. Tuinstra, L.G.M. and Haasnoot, W. Rapid Determination of Aflatoxin B1 in Dutch Feeding Stuffs by HPLC and Post-Column Derivatization J. CHROMATOG. 282, 457-462 (1983)
  74. Wells, C.E., Juenge, E.C., Furman, W.B. Simultaneous Assay of Thioridazine and its Major Metobolites in Plasma of Single Dosage Levels with a Novel Report of Two Ring Sulfoxides of Thioridazine J. PHARM. SCI. 72(6), 622-625 (1983)
  75. Miles, C.J., Moye, A., Scherer, S.J. A Simplified High Performance Liquid Chromatographic Residue Procedure for the Determination of Glyphosate Herbicide and (Aminomethyl) Phosphonic Acid in Fruits and Vegetables, Employing Post-Column Fluorogenic Labeling, J. AGR. FOOD CHEM. 31, 69-72 (1983)
  76. Tamura, Z. and Yazawa, K. Post-Column Oxidation of Reduced Biopterins for Fluorescence Detection. J. CHROMATOG. 254, 327-331 (1983)
  77. Birks, J.W. and Sigvardson, K.W. Peroxyoxalate Chemiluminescence Detection of Polycyclic Aromatic Hydrocarbons in Liquid Chromatography. ANAL. CHEM. 55, 432-435 (1983)
  78. Edlund, P.O., Westerland, D., Carlquist, J. Determination of Artesunate and Dihydroartemisinine in Plasma by Liquid Chromatography with Post-Column Derivatization and UV Detection. ACTA PHARM. SCI., 21, 223-234 (1984)
  79. Marsh, K.C., Sternson, L.A., Repta, A.J. Post-Column Reaction Detector for Platinum (II) Antineoplastic Agents. ANAL. CHEM. 56, 491-497 (1984)
  80. Hill, K.M., Hollowell, R.H., DalCortivo, L.A. Determination of N-Methylcarbamate Pesticides in Well Water by Liquid Chromatography with Post-Column Fluorescence Derivatization ANAL. CHEM. 56, 2465-2468 (1984)
  81. Vanderslice, J.T. and Higgs, D.J. HPLC Analysis with Fluorometric Detection of Vitamin C in Food Samples. J. CHROMATOG. SCI. 22, 485-489 (1984)
  82. Seki, T. and Yamaguchi, Y. Fluorimetric Determination of Catecholamines using Glycylglycine as the Reagent for Post-Column Derivatization. J. CHROMATOG. 287, 407-412 (1984)
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  84. Wada, A., Bonoshita, M., Tanaka, Y., Hibi, K. A Study of a Reaction System for Organic Acid Analysis using a pH Indicator or Post-Column Reagent. J. CHROMATOG. 291, 111-118 (1984)
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  86. Miyaguchi, K., Honda K., lmai, K. Sub-Picomol Chemiluminescence Detection of Dns-Amino Acids, Separated by HPLC with Gradient Elution. J. CHROMATOG. 303, 173-176 (1984)
  87. Mellbin, G. and Smith, B.E.F. Trace Determination of Aliphatic Amines using HPLC with Chemiluminescence Excitation and Photon Counting. J. CHROMATOG. 312, 203-210 (1984)
  88. Koziol, T., Grayeski, M.L., Weinberger, R. Approaches for the Determination of Trace Levels of Steroids in Blood Plasma by L.C. with Peroxyoxalate Chemiluminescence Detection. J. CHROMATOG. 317, 355-368, (1984)
  89. Dong, M.W. and DiCesare, J. Amino Acid Analysis by L.C. LIQ. CHROMATOG. 1(4), 222-228 (1984)
  90. Vrátný, P., Brinkman, U.A. Th., Frei, R.W. Comparative Study of Post-Column Reaction for the Detection of Saccharides in Liquid Chromotography. ANAL. CHEM. 57, 224-229 (1985)
  91. Dong, M.W. and Gant, J.R. High Speed Liquid Chromatographic Analysis of Amino Acids by Post-Column Sodium Hypochlorite-O-Phthalaldehyde Reaction. J. CHROMATOG. 327, 17-25 (1985)
  92. Kimura, M. and Itokawa, Y. Determination of Thiamine and its Phosphate Esters in Human and Rat Blood by HPLC with Post-Column Derivatization. J. CHROMATOG. 332, 181-188, (1985)
  93. Shi, R.J.Y., Gee, W.L., Williams, A.L., Benet, L.Z., Lin, E.T. Ion-Pair Liquid Chromotographic Analysis of Phenylpropanolamine in plasma and Urine by Post-Column Derivatization with O-Phthalaldehyde. J. LIQUID CHROMATOG. 8(8), 1489-1500 (1985)
  94. lmai, K. and Weinberger, R. Aryl Oxalate Chemiluminescence Detection for High-Performance Liquid Chromatography. TRAC. 4(7), 170-175 (1985)
  95. Thiel, P.G., Stockenstrom, S., Gathercole, P.S. Aflatoxin Analysis by Reverse Phase HPLC using Post-Column Derivatization for Enhancement of Fluorescence. J. LIQ. CHROMATOG. 9(1), 103-112 (1986)
  96. Effkemann, S., Pinkernell, U., Neumuller, R., Schwan, H., Engelhardt, H., Karst, U. Liquid Chromatographic Simultaneus Determination of Peroxycarboxylic Acids Using Postcolumn Derivatization, ANAL. CHEM. 70(18), 3857-3862 (1998)
  97. Koshiishi, I., Mamura, Y., Imanari, T., Simultaneous determination of vitamin C and its carbamylated derivatives by high-performance liquid chromatography with post-column derivatization. J. CHROMATOG. A. 806, 340-344 (1998)
  98. Edder, P., Cominoli, I., Corvi, C. Determination of streptomycin residues in food by solid-phase extraction and liquid chromatography with post-column derivatization and fluorometric detection. J. CHROMATOG. A. 830, 345-351 (1999)
  99. Diehl, G. and Karst, U. Post-column oxidative derivatization for the liquid chromatographic determination of phenothiazines. J. CHROMATOG. A. 890, 281-287 (2000)
  100. Michels, J.J. Improved measurement of formaldehyde in water-soluble polymers by high-performance liquid chromatography coupled with post-column reaction detection. J. CHROMATOG. A. 914, 123-129 (2001)
  101. Pakin, C., Bergaentzlé, M., Hubscher, V., Aoudé-Werner, D., Hasselmann, C. Fluorimetric determination of pantothenic acid in foods by liquid chromatography with post-column derivatization. J. CHROMATOG. A., 1035, 87-95 (2004)
  102. Maudens, K., Zhang, G-F., Lambert, W. Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection. J. CHROMATOG. A. 1047, 85-92 (2004)
 
 

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