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Economical Post Column Derivatization Kit (EPOCOD)

 

Economical Post Column Derivatization Kit (EPOCOD) is an economical and compact unit placed between the HPLC column and the detector designed to facilitate the convenient mixing of the analyte containing eluent from the HPLC column with chemical derivatization agents. The kit includes a PTFE knitted reactor coil, a column jacket for temperature control and a “T” connector for convenient mixing of the analyte with post column chemical derivatization agents.

A standard EPOCOD comes with a knitted reactor coil 5 meters long x 0.5 mm ID in a 4” long column jacket (EP B-4-5-50).   Other combinations of knitted reactor coils and column jackets can be provided based on your specific analytical needs.  A water circulator is used in conjunction with the column jacket to control the temperature of the post column chemical derivatization reaction. 

 

EPOCOD Economical Post Column Derivatization Kit (EPOCOD)

 

EPOCOD Catalog Numbers and Description:

Catalog No. Knitted Reactor Coil Length (M) ID (mm) Nominal Void Volume (mL) Recommended Column Jacket
EP B-4-5-25 KRC 5-25
5
0.25
0.25
CJB-4
EP C-10-10-25 KRC 10-25
10
0.25
0.5
CJC-10
EP C-10-15-25 KRC 15-25
15
0.25
0.75
CJC-10
EP C-10-20-25 KRC 20-25
20
0.25
1
CJC-10
EP C-10-25-25 KRC 25-25
25
0.25
1.25
CJC-10
EP B-4-5-50* KRC 5-50
5
0.5
1
CJB-4
EP C-10-10-50 KRC 10-50
10
0.5
2
CJC-10
EP C-10-15-50 KRC 15-50
15
0.5
3
CJC-10
EP C-10-20-50 KRC 20-50
20
0.5
4
CJC-10
EP C-10-25-50 KRC 25-50
25
0.5
5
CJC-10
Economical Post Column Derivatization Kit (EPOCOD)

* Standard

 

Bibliography

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  76. Tamura, Z. and Yazawa, K. Post-Column Oxidation of Reduced Biopterins for Fluorescence Detection. J. CHROMATOG. 254, 327-331 (1983)
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  78. Edlund, P.O., Westerland, D., Carlquist, J. Determination of Artesunate and Dihydroartemisinine in Plasma by Liquid Chromatography with Post-Column Derivatization and UV Detection. ACTA PHARM. SCI., 21, 223-234 (1984)
  79. Marsh, K.C., Sternson, L.A., Repta, A.J. Post-Column Reaction Detector for Platinum (II) Antineoplastic Agents. ANAL. CHEM. 56, 491-497 (1984)
  80. Hill, K.M., Hollowell, R.H., DalCortivo, L.A. Determination of N-Methylcarbamate Pesticides in Well Water by Liquid Chromatography with Post-Column Fluorescence Derivatization ANAL. CHEM. 56, 2465-2468 (1984)
  81. Vanderslice, J.T. and Higgs, D.J. HPLC Analysis with Fluorometric Detection of Vitamin C in Food Samples. J. CHROMATOG. SCI. 22, 485-489 (1984)
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  88. Koziol, T., Grayeski, M.L., Weinberger, R. Approaches for the Determination of Trace Levels of Steroids in Blood Plasma by L.C. with Peroxyoxalate Chemiluminescence Detection. J. CHROMATOG. 317, 355-368, (1984)
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  90. Vrátný, P., Brinkman, U.A. Th., Frei, R.W. Comparative Study of Post-Column Reaction for the Detection of Saccharides in Liquid Chromotography. ANAL. CHEM. 57, 224-229 (1985)
  91. Dong, M.W. and Gant, J.R. High Speed Liquid Chromatographic Analysis of Amino Acids by Post-Column Sodium Hypochlorite-O-Phthalaldehyde Reaction. J. CHROMATOG. 327, 17-25 (1985)
  92. Kimura, M. and Itokawa, Y. Determination of Thiamine and its Phosphate Esters in Human and Rat Blood by HPLC with Post-Column Derivatization. J. CHROMATOG. 332, 181-188, (1985)
  93. Shi, R.J.Y., Gee, W.L., Williams, A.L., Benet, L.Z., Lin, E.T. Ion-Pair Liquid Chromotographic Analysis of Phenylpropanolamine in plasma and Urine by Post-Column Derivatization with O-Phthalaldehyde. J. LIQUID CHROMATOG. 8(8), 1489-1500 (1985)
  94. lmai, K. and Weinberger, R. Aryl Oxalate Chemiluminescence Detection for High-Performance Liquid Chromatography. TRAC. 4(7), 170-175 (1985)
  95. Thiel, P.G., Stockenstrom, S., Gathercole, P.S. Aflatoxin Analysis by Reverse Phase HPLC using Post-Column Derivatization for Enhancement of Fluorescence. J. LIQ. CHROMATOG. 9(1), 103-112 (1986)
  96. Effkemann, S., Pinkernell, U., Neumuller, R., Schwan, H., Engelhardt, H., Karst, U. Liquid Chromatographic Simultaneus Determination of Peroxycarboxylic Acids Using Postcolumn Derivatization, ANAL. CHEM. 70(18), 3857-3862 (1998)
  97. Koshiishi, I., Mamura, Y., Imanari, T., Simultaneous determination of vitamin C and its carbamylated derivatives by high-performance liquid chromatography with post-column derivatization. J. CHROMATOG. A. 806, 340-344 (1998)
  98. Edder, P., Cominoli, I., Corvi, C. Determination of streptomycin residues in food by solid-phase extraction and liquid chromatography with post-column derivatization and fluorometric detection. J. CHROMATOG. A. 830, 345-351 (1999)
  99. Diehl, G. and Karst, U. Post-column oxidative derivatization for the liquid chromatographic determination of phenothiazines. J. CHROMATOG. A. 890, 281-287 (2000)
  100. Michels, J.J. Improved measurement of formaldehyde in water-soluble polymers by high-performance liquid chromatography coupled with post-column reaction detection. J. CHROMATOG. A. 914, 123-129 (2001)
  101. Pakin, C., Bergaentzlé, M., Hubscher, V., Aoudé-Werner, D., Hasselmann, C. Fluorimetric determination of pantothenic acid in foods by liquid chromatography with post-column derivatization. J. CHROMATOG. A., 1035, 87-95 (2004)
  102. Maudens, K., Zhang, G-F., Lambert, W. Quantitative analysis of twelve sulfonamides in honey after acidic hydrolysis by high-performance liquid chromatography with post-column derivatization and fluorescence detection. J. CHROMATOG. A. 1047, 85-92 (2004)
 
 

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