ERLIC HPLC Columns

Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) utilizes both hydrophilic interaction and electrostatic forces. By adjusting the pH, salt type, salt concentration, and organic solvent compositions in the mobile phase, one can achieve isocratic separation of a mixture of charged analytes, e.g., peptides, amino acids, and nucleotides. 

An ion-exchange column that has the SAME charge as the analytes is chosen. For example, the negatively charged nucleotides from the phosphate groups should use a cat-ion exchange column (stationary phase is negatively charged) in ERLIC. When an ion-exchange column is operated under a high-percentage organic mobile phase, hydrophilic interaction dominates over electrostatic. Therefore, molecules can be retained through hydrophilic interaction even if they possess the same charge as the stationary phase.  The repulsion from the same charged stationary phase and analytes shorten the retention time, resulting in a shorter chromatography run.

Source:

Alpert, A. Electrostatic Repulsion Hydrophilic Interaction Chromatography for Isocratic Separation of Charged Solutes and Selective Isolation of Phosphopeptides. Anal. Chem. 2008, 80, 62-76.

A summary of this paper is available on the Columnex blog.

ERLIC
PolyLC PolyCAT A
PolyLC PolySULFOETHYL A
PolyLC PolyWAX LP