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PolyLC Protein HPLC ColumnsPolyLC Inc.

PolyLC Inc., in Columbia, MD is a specialist in biomolecule separations, e.g., proteins, peptides, nucleic acids, and complex carbohydrates. Their special weak cation-exchange column, PolyCAT A, is capable of separating protein variants, hemoglobin variants, monoclonal antibodies, and histones. PolySULFOETHYL A, a strong cation-exchange column, is applicable in multidimensional HPLC separations of peptide mixtures, disulfide-linked peptides, phosphopeptides, and the mapping of digested C-terminal fragments. PolyWAX LP is a weak anion-exchange column useful in the isocratic separation of acidic amino acids, peptides, proteins, and nucleic acids.

Hydrophobic Interaction Chromatography (HIC) of Proteins and Peptides

PolyLC has three HIC columns designed specifically for protein and peptide separations: PolyPROPYL A, PolyETHYL A and PolyMETHYL A, with relative hydrophobic characters of 100, 60, and 15 respectively. HIC has greater sensitivity than other modes to the location of a modification or residue difference, especially if it involves a nonpolar group. HIC's selectivity is generally superior to those of reversed phase for proteins and polypeptides large enough to have significant secondary or tertiary structures. These HIC columns are applicable in multidimensional protein purifications, polypeptide purifications, antibody characterizations, and QC analysis of proteins differing by a single residue or modified positional variants.

Hydrophilic Interaction Chromatography (HILIC)

PolyHYDROXYETHYL A is a HILIC column applicable into glycopeptide variant purification from tryptic digests, metabolite analysis, and other polar analyte separations. In the absence of organic solvent, PolyHYDROXYETHYL A operates in only Gel-Filtration Chromatography (GFC) mode. At pH 4.4, the poly(2-hydroxyethyl aspartamide) is a neutral zwitterion. Above pH 4.4, the net charge on the stationary phase is negative; below, positive.

Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC)

ERLIC permits some separations that normally would require a gradient to be done isocratically. This technique also permits the selective isolation of phosphopeptides from a tryptic digest.  In ERLIC, the column is of the same charge as the sample solutes.  The mobile phase contains enough organic solvent so that hydrophilic interaction keeps the solutes on the column despite the electrostatic repulsion.  ERLIC can be performed with PolyCAT A, PolySULFOETHYL A, and PolyWAX LP columns.


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