HPLC Columns for Saccharides, Glycans, and Sugars

Recommended HPLC Columns

Amide and amino HPLC columns in HILIC mode are popular for the analysis of sugars and small oligosaccharides. Silica-based amide HPLC columns such as Unisol Amide or PolyGLYCOPLEX are a good choice for many applications.

Amino columns are equally popular if not more so. However, silica-based amino HPLC columns often suffer in inferior reproducibility and short column life time when used with aqueous mobile phase. Unison UK-Amino columns have high efficiency from its silica particles while maintains aqueous durable using its proprietary technology. Polymer-based amino columns (Jordi Gel Polyamino DVB) are stable in wide pH range of 1 -14, compatible with 100% water, and high sample loading capacity. The disadvantage of polymer-based amino columns is that they lack the efficiency of silica columns. The polymer-silica hybrid particles have the best of both polymer and silica. The hybrid material amino columns, Durashell NH2 and Chromenta EP-NH2 both have high efficiency and 100% aqueous compatible in wide pH range of around 1.5 or 2.0 - 12.0.

Ion exclusion columns (e.g. Carbomix) are often used in organic acid, small-sugar, and alcohol analyses with 100% aqueous mobile phase. It can be a powerful technique in fermentation monitoring and bioethanol production. Sepax Carbomix Catalog

Weak ion-exchange is used for acidic sugar analyses, and for neutral sugar separations under certain conditions. Then, the mixed-mode columns like Obelisc N can be useful by providing alternate selectivity. For large polysaccharides, size exclusion is the default method to determine molecular weight distribution. Jordi xStream H2O is the newest generation of SEC column for this purpose.

Saccharides

Saccharides is a synonym of carbohydrates. Saccharides are divided into four chemical groups: monosaccharide, disaccharide, oligosaccharide, and polysaccharide. Saccharides and their derivatives include many other important biomolecules that play key roles in the immune system, fertilization, pathogenesis, blood clotting, and development.

Glycans

Glycan refers to a polysaccharide or oligosaccharide. Glycan may also be used to refer to the carbohydrate portion of a glycoconjugate, such as a glycoprotein, glycolipid, or a proteoglycan. Glycans can be found attached to proteins as in glycoproteins and proteoglycans. They are generally found on the exterior surface of cells. O- and N-linked glycans are very common in eukaryotes but may also be found, although less commonly, in prokaryotes.

Sugars

Sugars commonly refers to monosaccharide or disaccharides. In food science and in many informal contexts, the term carbohydrate often means any food that is particularly rich in starch (such as cereals, bread and pasta) or sugar (such as candy, jams and desserts).

Normal Phase
Polymer-silica hybrid particles
Bonna-Agela Durashell NH2
Chromenta EP-NH2
Polymer-based particles
Jordi Gel Polyamino DVB
Jordi Gel Polyamino Sugars
Silica-based particles
Bonna-Agela Promosil NH2
Bonna-Agela Unisol Amide
Bonna-Agela Venusil XBP NH2
COSMOSIL NH2-MS
COSMOSIL SUGAR-D
Imtakt Unison UK-Amino
PolyLC PolyGLYCOPLEX

Ion Exclusion
Chromenta Sugar H+
Chromenta Sugar Ca++
MN Nucleogel Sugar 810 Ca
MN Nucleogel Sugar 810 H
MN Nucleogel Sugar Ca
MN Nucleogel Sugar Na
MN Nucleogel Sugar Pb
Sepax Carbomix Ca-NP10
Sepax Carbomix H-NP10
Sepax Carbomix K-NP10
Sepax Carbomix Na-NP10
Sepax Carbomix Pd-NP10

Ion-Exchange
COSMOSIL DEAE
PolyLC PolyWAX

Mixed Mode ( IEX + Normal Phase)
SIELC Obelisc N

Size Exclusion Chromatography (GFC)
Jordi xStream H2O